pLSU1/t35s[TMV]:mNeonGreen-CI{PDK-198}:NosT
(Plasmid #212176)

• Purpose: This binary vector expresses mNeonGreen containing the catalase (PDK) intron (390 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTR
• Depositing Lab: Wayne Curtis
• Publication: Curtis lab plasmids (unpublished)

Backbone

• Vector backbone: pLSU1
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tomato codon optimized version of mNeonGreen-I{PDK-198}
• Species: Synthetic
• Insert Size (bp): 900
• Mutation: mNeonGreen codon-optimized for tomato and PDK intron added at bp 198, eliminates expression in Agrobacterium, cryptic intron site in mNG removed
• Promoter: truncated 35sCaMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pLSU1_t35s_[TMV]_mNeonGreen-CI{PDK-198}_NosT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry