pLSU1/t35s[TMV]:mNeonGreen-CI{PIV-216}:NosT
(Plasmid #212173)

• Purpose: This binary vector expresses mNeonGreen containing the PIV intron (216 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTR
• Depositing Lab: Wayne Curtis
• Publication: Curtis lab plasmids (unpublished)

Backbone

• Vector backbone: pLSU1
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tomato codon optimized version of mNeonGreen-I{PIV-216}
• Species: Synthetic
• Insert Size (bp): 900
• Mutation: mNeonGreen codon-optimized for tomato and PIV intron added at bp 216, eliminates expression in Agrobacterium, cryptic intron site in mNG removed
• Promoter: truncated 35sCaMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pLSU1_t35s_[TMV]_mNeonGreen-CI{PIV-216}_NosT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry