pLSU1/t35s[TMV]:mNeonGreen-I{PDK-198}:NosT
(Plasmid #212170)

• Purpose: This binary vector expresses mNeonGreen containing the castor bean catalase (PDK) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
• Depositing Lab: Wayne Curtis
• Publication: Curtis lab plasmids (unpublished)

Backbone

• Vector backbone: pLSU1
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tomato codon optimized version of mNeonGreen-I{PDK-198}
• Species: Synthetic
• Insert Size (bp): 900
• Mutation: mNeonGreen codon-optimized for tomato and PDK intron added at bp 198, eliminates expression in Agrobacterium
• Promoter: truncated 35sCaMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pLSU1_t35s_[TMV]_mNeonGreen-I{PDK-198}_NosT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry