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Addgene

GFP-C1(2)delta
(Plasmid #21216)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21216 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4596
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP-N2-PKCdelta-C1
  • Alt name
    PKCdelta
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    414
  • Mutation
    C1(2) domains of PKC delta (rat) (Lab plasmid WC0001)
  • Entrez Gene
    Prkcd (a.k.a. Pkcd)
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-C1(2)delta was a gift from Tobias Meyer (Addgene plasmid # 21216 ; http://n2t.net/addgene:21216 ; RRID:Addgene_21216)

  • For your References section:

    Control of astrocyte Ca(2+) oscillations and waves by oscillating translocation and activation of protein kinase C. Codazzi F, Teruel MN, Meyer T. Curr Biol. 2001 Jul 24. 11(14):1089-97. 10.1016/S0960-9822(01)00326-8 PubMed 11509231
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