pDV02
(Plasmid
#212156)
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PurposePlasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 212156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMB1
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Vector typeBacterial Expression
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Selectable markersSpectinomycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesecondary alcohol dehydrogenase
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SpeciesCandida parapsilosis
- Promoter PgyrA
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Developing a nanopore sequencing workflow for protein engineering applications (DOI: 10.1021/acssynbio.3c00087)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDV02 was a gift from Kang Zhou (Addgene plasmid # 212156 ; http://n2t.net/addgene:212156 ; RRID:Addgene_212156) -
For your References section:
Developing a Nanopore Sequencing Workflow for Protein Engineering Applications. Tan DZJ, Fung V, Sun T, Tian K, Li Z, Zhou K. ACS Synth Biol. 2023 Jul 21;12(7):2041-2050. doi: 10.1021/acssynbio.3c00087. Epub 2023 Jul 4. 10.1021/acssynbio.3c00087 PubMed 37403232