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Addgene

pDV02
(Plasmid #212156)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212156 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMB1
  • Vector type
    Bacterial Expression
  • Selectable markers
    Spectinomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    secondary alcohol dehydrogenase
  • Species
    Candida parapsilosis
  • Promoter PgyrA

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Developing a nanopore sequencing workflow for protein engineering applications (DOI: 10.1021/acssynbio.3c00087)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDV02 was a gift from Kang Zhou (Addgene plasmid # 212156 ; http://n2t.net/addgene:212156 ; RRID:Addgene_212156)
  • For your References section:

    Developing a Nanopore Sequencing Workflow for Protein Engineering Applications. Tan DZJ, Fung V, Sun T, Tian K, Li Z, Zhou K. ACS Synth Biol. 2023 Jul 21;12(7):2041-2050. doi: 10.1021/acssynbio.3c00087. Epub 2023 Jul 4. 10.1021/acssynbio.3c00087 PubMed 37403232