PHB1
(Plasmid #212153)

• Purpose: For PHB production in E. coli (PT7-pMB1).
• Depositing Lab: Kang Zhou
• Publication: Harnessing xylitol utilization in Escherichia coli for antibiotic-free plasmid and recombinant protein production. (unpublished)

Backbone

• Vector backbone: pMB1
• Vector type: Bacterial Expression
• Selectable markers: Chloramphenicol

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
• Species: Cupriavidus necator
• Promoter: PT7

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Supplemental Documents:
  • PHB1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry