pXU01
(Plasmid
#212151)
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PurposePlasmid expressing xylitol dehydrogenase under inducible promoter PT7 control. Plasmid confers the ability to use xylitol as major carbon source to E. coli.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 212151 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMB1
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Vector typeBacterial Expression
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Selectable markersSpectinomycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namexylitol dehydrogenase
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SpeciesE. coli
- Promoter PT7
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Tag
/ Fusion Protein
- 6x His (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Producing aromatic amino acid from corn husk by using polyols as intermediates (DOI: 10.1016/j.biomaterials.2022.121661)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXU01 was a gift from Kang Zhou (Addgene plasmid # 212151 ; http://n2t.net/addgene:212151 ; RRID:Addgene_212151) -
For your References section:
Producing aromatic amino acid from corn husk by using polyols as intermediates. Fung V, Xiao Y, Tan ZJD, Ma X, Zhou JFJ, Panda S, Yan N, Zhou K. Biomaterials. 2022 Aug;287:121661. doi: 10.1016/j.biomaterials.2022.121661. Epub 2022 Jul 1. 10.1016/j.biomaterials.2022.121661 PubMed 35842981