GFP-C2gamma
(Plasmid #21215)

• Depositing Lab: Tobias Meyer
• Publication: Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5429
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP-pcDNA3-PKCgamma-c2
• Alt name: PKCgamma
• Species: R. norvegicus (rat)
• Insert Size (bp): 1114
• Mutation: The C2-domain from PKCgamma (aa170-260) plus flanking sequence. This insert is thus amino acids 158-284 from PKCgamma (rat). (Lab plasmid WC 0005)
• Entrez Gene: Prkcg (a.k.a. PKC, PKCI, Prkc, Prkcc, RATPKCI)
• Tag / Fusion Protein:
  • GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ApaI (unknown if destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry