pGU08
(Plasmid
#212149)
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PurposePlasmid expressing catalase-peroxidase and D-amino acid dehydrogenase under constitutive promoter PgyrA control. Best E. coli glycine utilization plasmid.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 212149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMB1
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Vector typeBacterial Expression
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Selectable markersSpectinomycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecatalase-peroxidase, D-amino acid dehydrogenase
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SpecieskatG: E. coli, dadA6: Cupriavidus necator
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Entrez GenekatG (a.k.a. b3942, ECK3934)
- Promoter PgyrA
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Improving glycine utilization in Escherichia coli (10.1016/j.bej.2023.108834)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGU08 was a gift from Kang Zhou (Addgene plasmid # 212149 ; http://n2t.net/addgene:212149 ; RRID:Addgene_212149)