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Addgene

pGU06
(Plasmid #212148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMB1
  • Vector type
    Bacterial Expression
  • Selectable markers
    Spectinomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase, tyrosine aminotransferase
  • Species
    S. cerevisiae (budding yeast); Agx1: S. cerevisiae; alaA, tyrB: E. coli
  • Entrez Gene
    AGX1 (a.k.a. YFL030W)
  • Entrez Gene
    alaA (a.k.a. b2290, ECK2284, yfbQ)
  • Entrez Gene
    tyrB (a.k.a. b4054, ECK4046)
  • Promoter PgyrA

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Improving glycine utilization in Escherichia coli (10.1016/j.bej.2023.108834)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGU06 was a gift from Kang Zhou (Addgene plasmid # 212148 ; http://n2t.net/addgene:212148 ; RRID:Addgene_212148)
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