INT_Cargo_T7_gfp
(Plasmid
#212136)
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PurposeCargo plasmid for integrating PT7 expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 212136 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSC101ts
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Vector typeBacterial Expression
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Selectable markersAmpicillin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namegreen fluorescent protein
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SpeciesAequorea victoria
- Promoter PT7
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Producing aromatic amino acid from corn husk by using polyols as intermediates (DOI: 10.1016/j.biomaterials.2022.121661)Modified from Addgene #160732
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
INT_Cargo_T7_gfp was a gift from Kang Zhou (Addgene plasmid # 212136 ; http://n2t.net/addgene:212136 ; RRID:Addgene_212136) -
For your References section:
Producing aromatic amino acid from corn husk by using polyols as intermediates. Fung V, Xiao Y, Tan ZJD, Ma X, Zhou JFJ, Panda S, Yan N, Zhou K. Biomaterials. 2022 Aug;287:121661. doi: 10.1016/j.biomaterials.2022.121661. Epub 2022 Jul 1. 10.1016/j.biomaterials.2022.121661 PubMed 35842981