pETSUMO2_Vitiosangium-bGSDM
(Plasmid #212127)

• Purpose: For expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.
• Depositing Lab: Philip Kranzusch
• Publication: Johnson et al Science. 2022 Jan 14;375(6577):221-225. doi: 10.1126/science.abj8432. Epub 2022 Jan 13.

Backbone

• Vector backbone: pETSUMO2
• Backbone manufacturer: custom
• Backbone size w/o insert (bp): 5932
• Total vector size (bp): 6736
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Vitiosangium bGSDM
• Alt name: Vitiosangium bacterial gasdermin
• Species: Vitiosangium sp. GDMCC 1.1324
• Insert Size (bp): 804
• Mutation: N-terminal methionine deleted and replaced by single serine scar after SUMO2 tag cleavage.
• GenBank ID: PZOX01000012.1
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis-SUMO2 (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GACGGGCAACCAATCAATGAAACAG
• 3′ sequencing primer: TATGCTAGTTATTGCTCAGCGGTG

Resource Information

• Supplemental Documents:
  • pETSUMO2_Vitiosangium-bGSDM.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry