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5'-3xFLAG Sap30bp+exon_siResist minimal CMV pCDNA5
(Plasmid #212085)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212085 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    5'-3xFLAG minimal CMV pCDNA5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sap30bp+exon(48nt)_siResist
  • Alt name
    NP_065229.2
  • Species
    M. musculus (mouse)
  • Mutation
    TCTAACCATCTGCAGGACA
  • Entrez Gene
    Sap30bp (a.k.a. Hcngp, 2700016D05Rik)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5'-3xFLAG Sap30bp+exon_siResist minimal CMV pCDNA5 was a gift from Benjamin Blencowe (Addgene plasmid # 212085 ; http://n2t.net/addgene:212085 ; RRID:Addgene_212085)

  • For your References section:

    Systematic analysis of alternative exon-dependent interactome remodeling reveals multitasking functions of gene regulatory factors. Roth JF, Braunschweig U, Wu M, Li JD, Lin ZY, Larsen B, Weatheritt RJ, Gingras AC, Blencowe BJ. Mol Cell. 2023 Dec 7;83(23):4222-4238.e10. doi: 10.1016/j.molcel.2023.10.034. 10.1016/j.molcel.2023.10.034 PubMed 38065061
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