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Addgene

pBbS8c_ddCpf1_final
(Plasmid #212040)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212040 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSC101
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ddCpf1
  • Species
    Synthetic
  • Promoter L-arabinose-inducible promoter PBAD

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbS8c_ddCpf1_final was a gift from Quanjiang Ji (Addgene plasmid # 212040 ; http://n2t.net/addgene:212040 ; RRID:Addgene_212040)
  • For your References section:

    BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases. Zhang H, Ma J, Wu Z, Chen X, Qian Y, Chen W, Wang Z, Zhang Y, Zhu H, Huang X, Ji Q. Nat Commun. 2024 Jan 27;15(1):825. doi: 10.1038/s41467-024-45114-4. 10.1038/s41467-024-45114-4 PubMed 38280845