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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN2
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4724
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePKC gamma
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SpeciesR. norvegicus (rat)
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Insert Size (bp)2090
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MutationPKC gamma (rat). (Lab plasmid WC 0013)
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Entrez GenePrkcg (a.k.a. PKC, PKCI, Prkc, Prkcc, RATPKCI)
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Tag
/ Fusion Protein
- GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-N2-PKCgamma was a gift from Tobias Meyer (Addgene plasmid # 21204 ; http://n2t.net/addgene:21204 ; RRID:Addgene_21204) -
For your References section:
Green fluorescent protein (GFP)-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol signaling in living cells. Oancea E, Teruel MN, Quest AF, Meyer T. J Cell Biol. 1998 Feb 9. 140(3):485-98. 10.1083/jcb.140.3.485 PubMed 9456311