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Addgene

GFP-N2-PKCgamma
(Plasmid #21204)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21204 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    N2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4724
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PKC gamma
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2090
  • Mutation
    PKC gamma (rat). (Lab plasmid WC 0013)
  • Entrez Gene
    Prkcg (a.k.a. PKC, PKCI, Prkc, Prkcc, RATPKCI)
  • Tag / Fusion Protein
    • GFP

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-N2-PKCgamma was a gift from Tobias Meyer (Addgene plasmid # 21204 ; http://n2t.net/addgene:21204 ; RRID:Addgene_21204)
  • For your References section:

    Green fluorescent protein (GFP)-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol signaling in living cells. Oancea E, Teruel MN, Quest AF, Meyer T. J Cell Biol. 1998 Feb 9. 140(3):485-98. 10.1083/jcb.140.3.485 PubMed 9456311