pegRNA2_tevopreQ1
(Plasmid
#212039)
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PurposeBacterial expression of the pegRNA for genome editing in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 212039 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepegRNA
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SpeciesSynthetic
- Promoter J23119
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pegRNA2_tevopreQ1 was a gift from Quanjiang Ji (Addgene plasmid # 212039 ; http://n2t.net/addgene:212039 ; RRID:Addgene_212039) -
For your References section:
BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases. Zhang H, Ma J, Wu Z, Chen X, Qian Y, Chen W, Wang Z, Zhang Y, Zhu H, Huang X, Ji Q. Nat Commun. 2024 Jan 27;15(1):825. doi: 10.1038/s41467-024-45114-4. 10.1038/s41467-024-45114-4 PubMed 38280845