Sp_PE
(Plasmid
#212038)
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PurposeBacterial expression of the PE2 proteins for genome editing in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 212038 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep15a
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePE2
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SpeciesSynthetic
- Promoter L-arabinose-inducible promoter PBAD
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sp_PE was a gift from Quanjiang Ji (Addgene plasmid # 212038 ; http://n2t.net/addgene:212038 ; RRID:Addgene_212038) -
For your References section:
BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases. Zhang H, Ma J, Wu Z, Chen X, Qian Y, Chen W, Wang Z, Zhang Y, Zhu H, Huang X, Ji Q. Nat Commun. 2024 Jan 27;15(1):825. doi: 10.1038/s41467-024-45114-4. 10.1038/s41467-024-45114-4 PubMed 38280845