pcDNA3/mStayGold(c4)=UtrCH
(Plasmid #212020)

• Purpose: For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).
• Depositing Lab: Atsushi Miyawaki
• Publication: Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 1596
• Total vector size (bp): 6980
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UtrCH
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1596
• Promoter: CMV
• Tag / Fusion Protein:
  • mStayGold(c4) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: sp6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-2941917/v1 for ResearchSquare preprint.