pRSETB/mStayGold(c4)
(Plasmid #212018)

• Purpose: For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.
• Depositing Lab: Atsushi Miyawaki
• Publication: Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6.

Backbone

• Vector backbone: pRSETB
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 3600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mStayGold(c4)
• Species: Synthetic
• Insert Size (bp): 711
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: pRSET reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-2941917/v1 for ResearchSquare preprint.