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Addgene

LentiGuide-10X-CS1-3'-puro
(Plasmid #212011)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212011 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiGuide puro
  • Total vector size (bp) 10216
  • Modifications to backbone
    Change original gRNA scaffold into 10x Genomics provided gRNA scaffold with CS1
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA scaffold
  • gRNA/shRNA sequence
    gRNA scaffold for gRNA cloning and delivery through lentivirus
  • Species
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiGuide-10X-CS1-3'-puro was a gift from Danwei Huangfu (Addgene plasmid # 212011 ; http://n2t.net/addgene:212011 ; RRID:Addgene_212011)
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