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Addgene

pCS2-hOGDH
(Plasmid #212010)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212010 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcs2
  • Backbone manufacturer
    A kind gift by Dr. David Turner, The University of Michigan, Ann Arbor, MI
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 7167
  • Vector type
    Zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    oxoglutarate dehydrogenase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3072
  • Entrez Gene
    OGDH (a.k.a. AKGDH, E1k, E1o, KGD1, OGDC, OGDH-E1, OGDH2, OGDHD)
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Cla1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer Sp6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-hOGDH was a gift from Gaurav Varshney (Addgene plasmid # 212010)
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