PEW-pEF1A-inactEGFP
(Plasmid
#212009)
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PurposeCleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transduction
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 212009 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePEW-pEF1a-inactEGFP
- Backbone size w/o insert (bp) 7658
- Total vector size (bp) 8408
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameInactivated eGFP
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SpeciesSynthetic
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Insert Size (bp)714
- Promoter pEF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GTGGGTGGAGACTGAAGTTAGGCC
- 3′ sequencing primer GAGGAGAAAATGAAAGCCATACGGGAAGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byCatherine Wu Lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PEW-pEF1A-inactEGFP was a gift from Catherine Wu (Addgene plasmid # 212009 ; http://n2t.net/addgene:212009 ; RRID:Addgene_212009)
Map uploaded by the depositor.
