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PEW-pEF1A-inactEGFP
(Plasmid #212009)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212009 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PEW-pEF1a-inactEGFP
  • Backbone size w/o insert (bp) 7658
  • Total vector size (bp) 8408
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Inactivated eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    714
  • Promoter pEF1a

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTGGGTGGAGACTGAAGTTAGGCC
  • 3′ sequencing primer GAGGAGAAAATGAAAGCCATACGGGAAGC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Catherine Wu Lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PEW-pEF1A-inactEGFP was a gift from Catherine Wu (Addgene plasmid # 212009 ; http://n2t.net/addgene:212009 ; RRID:Addgene_212009)

  • For your References section:

    Rapid parallel reconstruction and specificity screening of hundreds of T cell receptors. Afeyan AB, Wu CJ, Oliveira G. Nat Protoc. (2024) 10.1038/s41596-024-01061-4
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