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pKLV2-U6gRNA5(TRIM25_5-2)-PGKpuroBFP-W
(Plasmid #211990)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211990 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKLV2-U6gRNA5(BbsI)-PGKpuroBFP-W
  • Backbone size w/o insert (bp) 8630
  • Total vector size (bp) 8650
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA targeting TRIM25
  • gRNA/shRNA sequence
    GGCGCAACAGGTCGCGAAC
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_005082.5
  • Entrez Gene
    TRIM25 (a.k.a. EFP, RNF147, Z147, ZNF147)
  • Promoter Human U6 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer AGATAATTAGAATTAATTTGACTG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKLV2-U6gRNA5(TRIM25_5-2)-PGKpuroBFP-W was a gift from Kosuke Yusa (Addgene plasmid # 211990 ; http://n2t.net/addgene:211990 ; RRID:Addgene_211990)

  • For your References section:

    RENGE infers gene regulatory networks using time-series single-cell RNA-seq data with CRISPR perturbations. Ishikawa M, Sugino S, Masuda Y, Tarumoto Y, Seto Y, Taniyama N, Wagai F, Yamauchi Y, Kojima Y, Kiryu H, Yusa K, Eiraku M, Mochizuki A. Commun Biol. 2023 Dec 28;6(1):1290. doi: 10.1038/s42003-023-05594-4. 10.1038/s42003-023-05594-4 PubMed 38155269
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