pDMN1λT1-trpA
(Plasmid
#211945)
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Purposein vitro transcription
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211945 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameT7A1-O1-λT1trpA
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SpeciesSynthetic
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Insert Size (bp)1420
- Promoter T7A1
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pDM_N1_400_T1trpA_sq
Please visit https://doi.org/10.1101/2023.11.02.565415 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDMN1λT1-trpA was a gift from Laura Finzi (Addgene plasmid # 211945 ; http://n2t.net/addgene:211945 ; RRID:Addgene_211945) -
For your References section:
Force and the α-C-terminal domains bias RNA polymerase recycling. Qian J, Wang B, Artsimovitch I, Dunlap D, Finzi L. bioRxiv 2023.11.02.565415 10.1101/2023.11.02.565415