L1E-1
(Plasmid #21193)

• Depositing Lab: Paul Khavari
• Publication: Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Backbone

• Vector backbone: LZRS-RfA
• Backbone size w/o insert (bp): 11100
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: STBL2
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Mek1
• Alt name: MAP2K1
• Alt name: ER-{alpha}
• Alt name: Esr1
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 2200
• Mutation: Mek1 R4F fused to ER*, constitutive active
• Entrez Gene: Esr1 (a.k.a. ER, ER-alpha, ERa, ERalpha, ESR, Estr, Estra, Nr3a1)
• Entrez Gene: MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attR (not destroyed)
• 3′ cloning site: attR (not destroyed)
• 5′ sequencing primer: TGGATACACGCCGCCCACGTG
• 3′ sequencing primer: ATCGTCGACCACTGTGCTGG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constitutive active protein has the following aa sequence deleted - ALQKKLEELELDEQQRKRLE, residues 31 to 51.