pNBU2_repA2
(Plasmid
#211911)
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Purpose(Empty Backbone) Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211911 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNBU2_erm-TetR-P1T_DP-GH023
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Modifications to backboneExchanged integron with a fragment from pKSH24 to make vector replicative in Bacteroidales.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNBU2_repA2 was a gift from Adam Arkin (Addgene plasmid # 211911 ; http://n2t.net/addgene:211911 ; RRID:Addgene_211911)
