Skip to main content
Addgene

pCS2-hOGDHL
(Plasmid #211909)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211909 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pC2p+
  • Backbone manufacturer
    A kind gift by Dr. David Turner, The University of Michigan, Ann Arbor, MI
  • Backbone size w/o insert (bp) 4104
  • Total vector size (bp) 7137
  • Vector type
    Zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    oxoglutarate dehydrogenase L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3033
  • GenBank ID
    NM_018245
  • Entrez Gene
    OGDHL (a.k.a. YOBELN)
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    A cDNA clone encoding for human OGDHL (NM_018245) was purchased from Origene Technologies, Inc. This clone harbors two rare variants (L511 and N573) and were changed back to WT amino acid P511 and D573 as per NCBI reference sequence.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-hOGDHL was a gift from Gaurav Varshney (Addgene plasmid # 211909)
Related items: