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Addgene

pDLterm
(Plasmid #211903)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211903 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDL
  • Backbone manufacturer
    Jores et al, 2024
  • Backbone size (bp) 9190
  • Modifications to backbone
    The 35S enhancer was inserted upstream of the 35S minimal promoter and the rbcS terminator was replaced with a Esp3I Golden Gate cassette
  • Vector type
    Plant Expression, Luciferase
  • Promoter 35S enhancer + 35S minimal promoter
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDLterm was a gift from Stan Fields (Addgene plasmid # 211903 ; http://n2t.net/addgene:211903 ; RRID:Addgene_211903)

  • For your References section:

    Arabidopsis and maize terminator strength is determined by GC content, polyadenylation motifs and cleavage probability. Gorjifard S, Jores T, Tonnies J, Mueth NA, Bubb K, Wrightsman T, Buckler ES, Fields S, Cuperus JT, Queitsch C. Nat Commun. 2024 Jul 12;15(1):5868. doi: 10.1038/s41467-024-50174-7. 10.1038/s41467-024-50174-7 PubMed 38997252
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