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Addgene

pAAV hSyn RSET-T-GECO1.WPRE
(Plasmid #211902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV hSyn
  • Backbone size w/o insert (bp) 4290
  • Total vector size (bp) 5628
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T-GECO1
  • Alt name
    T-GECO
  • Alt name
    TGECO
  • Species
    Synthetic
  • Insert Size (bp)
    1338
  • Promoter hSyn
  • Tag / Fusion Protein
    • RSET peptide (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gtttgtacaaaaaagcaggc
  • 3′ sequencing primer aattcccactttgtacaag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV hSyn RSET-T-GECO1.WPRE was a gift from Robert Campbell (Addgene plasmid # 211902 ; http://n2t.net/addgene:211902 ; RRID:Addgene_211902)

  • For your References section:

    Blue-shifted genetically encoded Ca(2+) indicator with enhanced two-photon absorption. Aggarwal A, Sunil S, Bendifallah I, Moon M, Drobizhev M, Zarowny L, Zheng J, Wu SY, Lohman AW, Tebo AG, Emiliani V, Podgorski K, Shen Y, Campbell RE. Neurophotonics. 2024 Apr;11(2):024207. doi: 10.1117/1.NPh.11.2.024207. 10.1117/1.NPh.11.2.024207 PubMed 38577628
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