Skip to main content
Addgene

GFP-pcDNA3-PKCgamma-cys1Acys1B
(Plasmid #21190)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21190 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5439
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PKCgamma-cys1Acys1B
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1089
  • Mutation
    PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamma). (Lab plasmid WC 0015)
  • Entrez Gene
    Prkcg (a.k.a. PKC, PKCI, Prkc, Prkcc, RATPKCI)
  • Tag / Fusion Protein
    • GFP

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-pcDNA3-PKCgamma-cys1Acys1B was a gift from Tobias Meyer (Addgene plasmid # 21190 ; http://n2t.net/addgene:21190 ; RRID:Addgene_21190)

  • For your References section:

    Green fluorescent protein (GFP)-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol signaling in living cells. Oancea E, Teruel MN, Quest AF, Meyer T. J Cell Biol. 1998 Feb 9. 140(3):485-98. 10.1083/jcb.140.3.485 PubMed 9456311
Related items:
Commonly requested with: