pGS97_EGFP(A206K)-CENP-T(1-242)-I3-01 AAVS donor
(Plasmid
#211866)
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PurposeCENP-T(1-242)-I3-01 transgene integration at AAVS1 safe harbor locus
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211866 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNM280
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Backbone manufacturerCheeseman Lab
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Vector typeCRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCENP-T(1-242)-I3-01
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SpeciesH. sapiens (human), Synthetic
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MutationN/A
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Entrez GeneCENPT (a.k.a. C16orf56, CENP-T, SSMGA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGS97_EGFP(A206K)-CENP-T(1-242)-I3-01 AAVS donor was a gift from Iain Cheeseman (Addgene plasmid # 211866 ; http://n2t.net/addgene:211866 ; RRID:Addgene_211866)