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pUPD2-HR1bR181A R185A-GFP-L (B3-B4)
(Plasmid #211857)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211857 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUPD2
  • Backbone manufacturer
    Diego Orzaez Lab
  • Backbone size w/o insert (bp) 2105
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HR1b
  • Species
    Synthetic
  • Mutation
    R181A R185A
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Rojas-Pierce Lab plasmid pMRP1179

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUPD2-HR1bR181A R185A-GFP-L (B3-B4) was a gift from Marcela Rojas-Pierce (Addgene plasmid # 211857 ; http://n2t.net/addgene:211857 ; RRID:Addgene_211857)

  • For your References section:

    Rapid depletion of target proteins in plants by an inducible protein degradation system. Huang L, Rojas-Pierce M. Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072. 10.1093/plcell/koae072 PubMed 38446628
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