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pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter
(Plasmid #211847)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211847 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDGB3_alpha2
  • Backbone manufacturer
    Diego Orzaez Lab
  • Backbone size w/o insert (bp) 6369
  • Vector type
    Plant Expression, Synthetic Biology
  • Selectable markers
    Rifamycin for Agrobacterium

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HR1b
  • Alt name
    Arabidopsis ROOT HAIR DEFECTIVE6 (RHD6)
  • Species
    A. thaliana (mustard weed)
  • Mutation
    R181A R185A
  • Promoter 35S
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Rojas-Pierce Lab plasmid pMRP1201, Design 98

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter was a gift from Marcela Rojas-Pierce (Addgene plasmid # 211847 ; http://n2t.net/addgene:211847 ; RRID:Addgene_211847)

  • For your References section:

    Rapid depletion of target proteins in plants by an inducible protein degradation system. Huang L, Rojas-Pierce M. Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072. 10.1093/plcell/koae072 PubMed 38446628
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