pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos
(Plasmid #211845)

• Purpose: Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.
• Depositing Lab: Marcela Rojas-Pierce
• Publication: Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.

Backbone

• Vector backbone: pDGB3_alpha1
• Backbone manufacturer: Diego Orzaez Lab
• Backbone size w/o insert (bp): 6367
• Vector type: Plant Expression, Synthetic Biology
• Selectable markers: Rifamycin for Agrobacterium

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: This plasmid is prone to recombination.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: LRR-NEL
• Species: Synthetic
• Mutation: C492A
• Promoter: pUBQ10
• Tags / Fusion Proteins:
  • mCherry (C terminal on insert)
  • HA (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: GAL4-VP16-GR
• Species: Synthetic
• Promoter: 6xUAS-35Smini
• Tags / Fusion Proteins:
  • Myc (N terminal on insert)
  • HA (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pDGB3_alpha1-pAtUBQ10-Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL C492A-mcherry-Tnos (242).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Rojas-Pierce Lab plasmid pMRP1192, Design 242