pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos
(Plasmid #211844)

• Purpose: Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.
• Depositing Lab: Marcela Rojas-Pierce
• Publication: Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.

Backbone

• Vector backbone: pDGB3_alpha1
• Backbone manufacturer: Diego Orzaez Lab
• Backbone size w/o insert (bp): 6367
• Vector type: Plant Expression, Synthetic Biology
• Selectable markers: Rifamycin for Agrobacterium

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LRR-NEL
• Species: Salmonella typhimurium
• Mutation: C492A
• Promoter: UBIQUITIN10
• Tags / Fusion Proteins:
  • 3xNLS-HA (N terminal on insert)
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)

Resource Information

• Supplemental Documents:
  • pDGB3_alpha1-pAtUBQ10-3xNLS-HA-LRR-NEL C492A-mcherry2-Tnos (Degron 225).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Rojas-Pierce Lab plasmid pMRP1183, Design 225