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pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos
(Plasmid #211841)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211841 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDGB3_alpha1
  • Backbone manufacturer
    Diego Orzaez Lab
  • Backbone size w/o insert (bp) 6367
  • Vector type
    Plant Expression, Synthetic Biology
  • Selectable markers
    Rifamycin for Agrobacterium

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LRR-NEL
  • Species
    Salmonella typhimurium
  • Promoter UBIQUITIN10
  • Tags / Fusion Proteins
    • 3xNLS-HA (N terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Rojas-Pierce Lab plasmid pMRP1191, Design 224

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos was a gift from Marcela Rojas-Pierce (Addgene plasmid # 211841 ; http://n2t.net/addgene:211841 ; RRID:Addgene_211841)

  • For your References section:

    Rapid depletion of target proteins in plants by an inducible protein degradation system. Huang L, Rojas-Pierce M. Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072. 10.1093/plcell/koae072 PubMed 38446628
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