pRRL-VinculinTS-S1033A
(Plasmid #211835)

• Purpose: Vinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
• Depositing Lab: Brenton Hoffman
• Publication: Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;120(50):e2316456120. doi: 10.1073/pnas.2316456120. Epub 2023 Dec 6.

Backbone

• Vector backbone: pRRL
• Backbone size w/o insert (bp): 6106
• Total vector size (bp): 11630
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VinculinTS-S1033A
• Alt name: VinTS-S1033A
• Species: G. gallus (chicken), Synthetic
• Insert Size (bp): 5524
• Mutation: mutated vinculin serine 1033 to alanine (S1033A), creates unphosphorylatable mutant
• Entrez Gene: VCL (a.k.a. VINC1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: GGTACAGTGCAGGGGAAAG
• 3′ sequencing primer: GTTAAGAATACCAGTCAATCTTTCAC

Resource Information

• Supplemental Documents:
  • prrl-vints_s1033a.gb
• A portion of this plasmid was derived from a plasmid made by: Martin Schwartz (Addgene #26019)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry