pUC19-link-mCherry-FLAG:cat
(Plasmid
#211831)
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PurposePlasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211831 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-FLAG-cat
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SpeciesSynthetic
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MutationNA
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains an IS1 prokaryotic transposable element that does not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19-link-mCherry-FLAG:cat was a gift from Peng Chen (Addgene plasmid # 211831 ; http://n2t.net/addgene:211831 ; RRID:Addgene_211831)
