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Addgene

pAc5.1C-FLuc-V5His6
(Plasmid #21183)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21183 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1C
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5371
  • Vector type
    Drosophila expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Dh5 alpha or XL1-blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Firefly Luciferase
  • Alt name
    FLuc
  • Insert Size (bp)
    1653

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Ac5 forward primer site
  • 3′ sequencing primer BGH reverse priming site
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1C-FLuc-V5His6 was a gift from Elisa Izaurralde (Addgene plasmid # 21183 ; http://n2t.net/addgene:21183 ; RRID:Addgene_21183)

  • For your References section:

    A crucial role for GW182 and the DCP1:DCP2 decapping complex in miRNA-mediated gene silencing. Rehwinkel J, Behm-Ansmant I, Gatfield D, Izaurralde E. RNA. 2005 Nov . 11(11):1640-7. 10.1261/rna.2191905 PubMed 16177138
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