pKERepPar
(Plasmid #211827)

• Purpose: Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.
• Depositing Lab: Anita Emmerstorfer-Augustin
• Publication: Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. doi: 10.1186/s12934-023-02280-2.

Backbone

• Vector backbone: pKRepPar
• Backbone size w/o insert (bp): 5219
• Total vector size (bp): 5929
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: 200 µg/mL Kanamycin for C. necator
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP
• Insert Size (bp): 720
• Promoter: T5

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGGGCTGACCGCGTCAGAC

Resource Information

• Supplemental Documents:
  • pKERepPar.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry