pLenti_mNeonGreen-blast-GACT
(Plasmid
#211822)
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PurposeLentiviral vector for fluorescence and barcoding of human cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211822 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin (Addgene # 211471)
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Backbone manufacturerEshleman Lab
- Backbone size w/o insert (bp) 13703
- Total vector size (bp) 9616
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Modifications to backboneRemoved Cas9, added M13F primer site and molecular barcode GGAACCTT
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameM13F and barcode GGAACCTT
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Insert Size (bp)29
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.04.15.537042 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_mNeonGreen-blast-GACT was a gift from James Eshleman (Addgene plasmid # 211822 ; http://n2t.net/addgene:211822 ; RRID:Addgene_211822) -
For your References section:
CRISPR-Cas9 for selective targeting of somatic mutations in pancreatic cancers. Teh SSK, Bowland K, Bennett A, Halper-Stromberg E, Skaist A, Tang J, Cai F, Macoretta A, Liang H, Kamiyama H, Wheelan S, Lin MT, Hruban RH, Scharpf RB, Roberts NJ, Eshleman JR. bioRxiv. 2023 Oct 10:2023.04.15.537042. doi: 10.1101/2023.04.15.537042. Preprint. 10.1101/2023.04.15.537042 PubMed 37131822