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Addgene

pKT-CNP-inact
(Plasmid #211804)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211804 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKT
  • Backbone size w/o insert (bp) 3641
  • Total vector size (bp) 4301
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    2',3'-cyclic nucleotide phosphodiesterase catalytic domain
  • Alt name
    CNP
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    660
  • Mutation
    H73L H153L
  • Entrez Gene
    Cnp (a.k.a. CNPF, CNPI, CNPII, Cnp1)
  • Promoter tet

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer ACCACTCCCTA GTAAAACGACGGCCAGT
  • 3′ sequencing primer GTAAAACGACGGCCAGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKT-CNP-inact was a gift from Emily Weinert (Addgene plasmid # 211804 ; http://n2t.net/addgene:211804 ; RRID:Addgene_211804)

  • For your References section:

    RNase I regulates Escherichia coli 2',3'-cyclic nucleotide monophosphate levels and biofilm formation. Fontaine BM, Martin KS, Garcia-Rodriguez JM, Jung C, Briggs L, Southwell JE, Jia X, Weinert EE. Biochem J. 2018 Apr 30;475(8):1491-1506. doi: 10.1042/BCJ20170906. 10.1042/BCJ20170906 PubMed 29555843
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