pLV-SFFV-Emerald-WPRE-UbC-MCS
(Plasmid
#211800)
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PurposeLentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHRsinUbEm
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameEmerald
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Alt nameEmGFP
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SpeciesA. victoria
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Insert Size (bp)717
- Promoter SFFV
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namemultiple cloning site
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SpeciesSynthetic
- Promoter UbC
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byVectorBuilder
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-SFFV-Emerald-WPRE-UbC-MCS was a gift from Richard Eva & Bart Nieuwenhuis (Addgene plasmid # 211800 ; http://n2t.net/addgene:211800 ; RRID:Addgene_211800)
