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Addgene

pRhaCAST
(Plasmid #211791)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211791 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZLrhaB2plus
  • Backbone manufacturer
    Silvia Cardona (Plasmid # 211787)
  • Backbone size w/o insert (bp) 13768
  • Total vector size (bp) 7792
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
  • Insert Size (bp)
    5800
  • Promoter Rhamnose-inducible

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRhaCAST was a gift from Silvia Cardona (Addgene plasmid # 211791 ; http://n2t.net/addgene:211791 ; RRID:Addgene_211791)
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