pDonorTp-fugfp
(Plasmid
#211790)
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PurposepDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211790 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDonorTp
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Backbone manufacturerSilvia Cardona (Plasmid # 211788)
Growth in Bacteria
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Bacterial Resistance(s)Trimethoprim
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namefuGFP
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XmaI (not destroyed)
- 3′ cloning site NdeI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note there is an IS5-like transposable element in this plasmid. Depositor confirms presence does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDonorTp-fugfp was a gift from Silvia Cardona (Addgene plasmid # 211790 ; http://n2t.net/addgene:211790 ; RRID:Addgene_211790)