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pET28a_LolT_C41S
(Plasmid #211789)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211789 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5340
  • Total vector size (bp) 6762
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lolt mutant
  • Alt name
    LolT_C41S
  • Species
    Synthetic
  • Insert Size (bp)
    1422
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACCTGTACTTCCAATCCAATcctgcactccctctctctg
  • 3′ sequencing primer ATCCGTTATCCACTTCCAATctagtgtttagaaatgacatgcccc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a_LolT_C41S was a gift from Yang Hai (Addgene plasmid # 211789 ; http://n2t.net/addgene:211789 ; RRID:Addgene_211789)

  • For your References section:

    Stereoselective Biocatalytic alpha-Deuteration of L-Amino Acids by a Pyridoxal 5'-Phosphate-Dependent Mannich Cyclase. Gao J, Zhou C, Hai Y. Chembiochem. 2023 Oct 1:e202300561. doi: 10.1002/cbic.202300561. 10.1002/cbic.202300561 PubMed 37779345
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