pDonorTp
(Plasmid
#211788)
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PurposepDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDonor_ShCAST_kanR
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Backbone manufacturerFeng Zhang (Plasmid #127924)
Growth in Bacteria
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Bacterial Resistance(s)Trimethoprim
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedhfr
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Insert Size (bp)671
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDonorTp was a gift from Silvia Cardona (Addgene plasmid # 211788 ; http://n2t.net/addgene:211788 ; RRID:Addgene_211788)