LLP791_PB_dCas9-SSSavi_BirA
(Plasmid #211786)

• Purpose: PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection
• Depositing Lab: Ryan Lister
• Publication: Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108.

Backbone

• Vector backbone: PB_CAH
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9, Spy, Snoop, aGCN4, AviTags
• Species: H. sapiens (human)
• Mutation: dCas9 D10A and H840A
• Promoter: pCAGG abd pEF1a
• Tag / Fusion Protein:
  • 3xHA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • LLP791_PB_dCas9-SSSavi_BirA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.07.01.498378v1 for bioRxiv preprint.