LLP618_αGCN4-KRAB-CO
(Plasmid
#211782)
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PurposeSunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211782 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLLP252 pEF1a-NLS-scFvGCN4-DNMT3a
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Backbone manufacturerRyan Lister (Addgene plasmid # 100941)
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Vector typeMammalian Expression
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameaGCN4-mCherry
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SpeciesH. sapiens (human)
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MutationLast 300 bp codon-optimised (CO) to detect mCherry expression distinct from gRNA selectable marker
- Promoter pEF1a and pSV40
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Tag
/ Fusion Protein
- 3xTy1 (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.07.01.498378v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LLP618_αGCN4-KRAB-CO was a gift from Ryan Lister (Addgene plasmid # 211782 ; http://n2t.net/addgene:211782 ; RRID:Addgene_211782) -
For your References section:
A modular dCas9-based recruitment platform for combinatorial epigenome editing. Swain T, Pflueger C, Freytag S, Poppe D, Pflueger J, Nguyen TV, Li JK, Lister R. Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108. 10.1093/nar/gkad1108 PubMed 38000387
