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Addgene

LLP784_SnoopCatcher-DNMT3A-CO
(Plasmid #211777)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211777 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LLP252 pEF1a-NLS-scFvGCN4-DNMT3a
  • Backbone manufacturer
    Ryan Lister (Addgene plasmid # 100941)
  • Vector type
    Mammalian Expression
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spy-EZH2
  • Species
    H. sapiens (human)
  • Mutation
    Last 300 bp codon-optimised (CO) to detect EZH2 expression distinct from endogenous mRNA
  • Promoter pEF1a and pSV40
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LLP784_SnoopCatcher-DNMT3A-CO was a gift from Ryan Lister (Addgene plasmid # 211777 ; http://n2t.net/addgene:211777 ; RRID:Addgene_211777)

  • For your References section:

    A modular dCas9-based recruitment platform for combinatorial epigenome editing. Swain T, Pflueger C, Freytag S, Poppe D, Pflueger J, Nguyen TV, Li JK, Lister R. Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108. 10.1093/nar/gkad1108 PubMed 38000387
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